LIAO Chang-neng, PAN Yong. Determination of Umbelliferone in of Traditional Zhuang Medicine[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 149-151.
LIAO Chang-neng, PAN Yong. Determination of Umbelliferone in of Traditional Zhuang Medicine[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 149-151.DOI:
HPLC测定壮药马蹄金中伞形花内酯的含量
摘要
目的: 建立马蹄金中伞形花内酯的含量测定方法。 方法: 采用VP-ODS C18(4.6 mm×150 mm
Objective:To establish the determination method of umbelliferone content in Dichondra repens. Method: VP-ODS Cl8(4.6 mm×150 mm
5 μm) column was used. The mobile phase was methanol-tetrahydrofuran-water solution (15∶85); the flow rate was 1.0 mL·min-1; the detection wavelength was at 325 nm. Result: The linear relation of hyperin was good in the range from 0.050 7 to 0.507 0 μg. The average recovery was 98.85%
RSD was 1.00%. Conclusion: The method established is accurate
sensitive and good in reproducibility. It can be applied to the quality control of D. repens.