XUE Ping, TANG Bin, CHEN Jian-wei, et al. Study on Inhibition of Alpha-glucoside and Antioxidation of from Leaves and Twigs of var [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 246-249.
XUE Ping, TANG Bin, CHEN Jian-wei, et al. Study on Inhibition of Alpha-glucoside and Antioxidation of from Leaves and Twigs of var [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 246-249.DOI:
Objective: Inhibition of alpha-glucosidase and antioxidation of different extracts from the branches and leaves of Taxus chinensis var mairei were studied in vitro to supply the separating base of the effective chemical components. Method: The inhibitory activities of α-glucoside were assayed in external.The antioxidant activities of these extracts were assessed bytwo complementary test systems
namely 1
1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power(FRAP) assay. Result: The ethyl acetate extract showed strong activity in the inhibitory activities of α-glucoside.(IC50 27.854 1 mg·L-1) The ethanol extract (IC50 29.215 5 mg·L-1)and n-butanol extract(IC50 38.913 0 mg·L-1) displayed lower activity. Petroleum ether fraction(IC50 128.729 5 mg·L-1) and water fraction(IC50 74.505 9 mg·L-1) have a little activity. The antioxidant activities:ethanol extract (DPPH IC50 6.370 7 mg·L-1
FRAP 0.678 mmol·g-1)>petroleum ether fraction. Conclusion: The components of inhibition α-glucoside could be testified in the ethyl acetate extract and n-butanol extract. Antioxidative components were in the ethyl acetate extract.