Objective:To establish content determination method of total fluorescent substances from Ligusticum chuanxiong

and optimize ultrasonic extraction technology of total fluorescent substances. Method: Fluorescence spectrophotometric method was adopted for determining the content of total fluorescent substances from L. chuanxiong.Influence of solid-liquid ratio

extraction time

kind and concentration of extraction solvent on extraction technology of total fluorescent substances was investigated by single factor test

ultrasound extraction technology parameters of total fluorescent substances were optimized by composite design

multiple linear regression and binomial fitting were used to evaluate relationship between independent and dependent variables

response surface methodology was used to optimize extraction process

prediction analysis was carried out by comparing the observed value and the predicted value. Result: Optimal technology conditions of ultrasonic extraction process were as followings:ultrasound extracted 53 min with 350 times the amount of 63% ethanol.The predictive extraction rate of total fluorescent substances from L. chuanxiong was 3.444%

bias between the observed value and the predicted value was 2.70%.Regression coefficients of binomial fitting complex model was as high as 0.953 2. Conclusion: This optimized ultrasonic extraction technology was simple with high precision and good predictability.Established content determination method was stable and feasible

it could provide a theoretical basis for quality control of L. chuanxiong.