Objective: To explore the inhibition effect of salvia extract on cultured neonatal rat hypertrophic cells induced by angiotensin Ⅱ(AngⅡ)and the related gene regulation to mRNA expression. Method: The Ang II-induced cardiac myocyte hypertrophy model was replicated

the neonatal rat cardiomyocytes in primary culture were randomly divided into normal control group

model group

valsartan (Val) in the control group

and salvia extract three different dose groups. There was any added drugs in the normal control group

and the final concentration was 10-6 g· L-1 AngⅡ in the model group

10-6g· L-1 Val in Val control group

and 10-4

10-3

10-2g· L-1 salvia extract in the other three groups. Myocardial cell counting and diameter were determined by the BI-2000 medical micrograph analyzing system

protein content was detected by the Bradford method

and protein kinase D1(PKD1) mRNA expression was analysed by the reverse transcription PCR (RT-PCR) method. Result: Compared with the normal control group

the number of myocardial cells in model group changed little

yet cardiomyocyte diameter

total protein content and PKD1 mRNA expression were significantly increased (P<0.05); compared with the model group

cardiomyocyte diameter

the total protein content and PKD1 mRNA expression of the Val control group were significantly lower (P<0.05); as well as the 10-3

10-2g· L-1 salvia extract groups (P<0.05). Conclusion: Salvia extract can significantly inhibit hypertrophy of cardiac myocyte induced by Ang II

which may be closely related to the mRNA expression regulation of PKD1.