HPLC程序可变波长法测定绞股蓝中芦丁、人参皂苷Rb和槲皮素

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HPLC程序可变波长法测定绞股蓝中芦丁、人参皂苷Rb和槲皮素

更新时间：2024-09-23

- HPLC程序可变波长法测定绞股蓝中芦丁、人参皂苷Rb和槲皮素
- Determination of Rutin,Ginsenoside Rb and Quercetin in by HPLC with Programmed Wavelength UV Detection
- 中国实验方剂学杂志 2013年19卷第3期页码：127-129
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- 纸质出版日期：2013
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廖秀英. HPLC程序可变波长法测定绞股蓝中芦丁、人参皂苷Rb和槲皮素[J]. 中国实验方剂学杂志, 2013,19(3):127-129.

LIAO Xiu-ying. Determination of Rutin,Ginsenoside Rb and Quercetin in by HPLC with Programmed Wavelength UV Detection[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 127-129.
廖秀英. HPLC程序可变波长法测定绞股蓝中芦丁、人参皂苷Rb和槲皮素[J]. 中国实验方剂学杂志, 2013,19(3):127-129. DOI：

LIAO Xiu-ying. Determination of Rutin,Ginsenoside Rb and Quercetin in by HPLC with Programmed Wavelength UV Detection[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 127-129. DOI：

摘要

目的: 建立绞股蓝药材中芦丁、人参皂苷Rb1和槲皮素的含量测定方法。方法: TSK ODS-100V(4.6 mm×250 mm

5 μm)色谱柱;流动相乙腈-0.1%磷酸(30∶70)

流速1.0 mL·min-1

柱温30 ℃

进样量10 μL

检测波长(0~14 min

257 nm;14~18 min

203 nm;18~30 min

257 nm)。结果: 芦丁、人参皂苷Rb1和槲皮素分别在0.171 2~1.284 0 μg、0.828 0~6.210 0 μg

0.204 8~1.525 7 μg线性关系良好

回归方程分别为Y=732 927.570 1X+1 523.016 4

r=0.9998、Y=158 991.787 4X+307.545 6

r=0.999 9

Y=745 728.515 6X+2 101.166 8(r=0.999 8)

平均加样回收率分别为97.33%(RSD 2.02%)

98.61%(RSD 1.70%)

98.38%(RSD 2.59%)。结论: 所建立的方法准确性高

重复性好

可作为绞股蓝药材的质量控制方法。

Abstract

Objective:To study and establish the quantitative analysis method for rutin

ginsenoside Rb1 and quercetin in Gynostemma pentapylla by HPLC with programmed wavelength UV detection. Method: HPLC was performed on TSK ODS-100V(4.6 mm×250 mm

5 μm)

the mobile phase consisted of acetonitrile and water(containing 0.1% phosphoric acid) at a flow rate of 1.0 mL·min-1.The column temperature was set at 30 ℃

and the eluate was detected by programmed wavelength(0-14 rnin

257 nm;14-18 min

203 nm;18-30 min

257 nm). Result: Quantitative analysis of HPLC showed that the linear ranges of rutin

ginsenoside Rb1 and quercetin were 0.171 2-1.284 0 μg(r=0.999 8)

0.828 0-6.210 0 μg(r=0.999 9)

0.204 8-1.525 7 μg(r=0.999 8) respectively.The average recoveries of rutin

ginsenoside Rb1 and quercetin were 97.33%(RSD=2.02%)

98.61%(RSD=1.70%)

98.38%(RSD=2.59%) respectively. Conclusion: The method set up is accurate

feasible and reliable

therefore available for the quality control of G. pentapylla.

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