Objective:To study the protective effects of Alpiniae Officinarum Rhizome

Alpiniae Katsumadai Semen and Galangae Fructus on stomach mucous membrane in rat stomach cold models

revealing the correlation between the volatile oil in these Chinese medicine and the efficacy of warming middle-jiao to dispel cold

explaining the scientific connotation of medication by boiling water extracts. Method: Two hundred and twenty rats were randomly divided into 22 groups: blank group

model groupⅠand Ⅱ

positive group

high and low dose volatile oil groups

high and low dose boiling water extract groups

high and low dose boiling water extract without volatile oil groups. All rats except blank group were fed cold drugs in the dose of 30 g·kg-1 twice a day for two days. Then they were given different volatile oil

boiling water extract and boiling water extract without volatile oil of the three kinds of Chinese medicines. The pathological changes of the stomach mucous membrane were observed under optical microscope. Result: The high and low dose volatile oil groups and high dose boiling water extract group and high dose boiling water extract without volatile oil group of Lesser Galangal Rhizome

all the groups of Katsumada Galangal Seed and Galanga Galangal Fruit could significantly improve the inflammatory cell infiltration(P<0.05 or P<0.01). The high dose volatile oil group

high dose boiling water extract group and high dose boiling water extract without volatile oil group of Lesser Galangal Rhizome

high and low dose volatile oil groups

high dose boiling water extract group

high and low dose boiling water extract without volatile oil groups of Katsumada Galangal Seed

high and low dose volatile oil groups

high and low dose boiling water extract groups

high dose boiling water extract without volatile oil group of Galanga Galangal Fruit could significantly improve glandular reduction or glandular destruction(P<0.05 or P<0.01). The high and low dose volatile oil groups

high and low dose boiling water extract groups of Lesser Galangal Rhizome

all the groups of Katsumada Galangal Seed

high and low dose volatile oil groups

high dose boiling water extract group

high dose boiling water extract without volatile oil group of Galanga Galangal Fruit could significantly improve mucosal congestion and edema(P<0.05 or P<0.01). Conclusion: The treatment groups of Lesser Galangal Rhizome

Katsumada Galangal Seed and Galanga Galangal Fruit all have the protection on stomach mucous membrane in rat stomach cold models

the main active ingredients are volatile oil

the boiling water extract and boiling water extract without volatile oil also contained active ingredient

the medication by boiling water extracts was scientific.