D101型大孔树脂纯化鸡骨草总黄酮的工艺优选

廖春燕; 杨欣绿

您当前的位置：

首页 >

文章列表页 >

D101型大孔树脂纯化鸡骨草总黄酮的工艺优选

更新时间：2024-01-04

- D101型大孔树脂纯化鸡骨草总黄酮的工艺优选
- Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin
- 中国实验方剂学杂志 2013年19卷第2期页码：38-40
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西科技大学(筹)科学基金项目(校科自1261103)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
廖春燕, 杨欣绿. D101型大孔树脂纯化鸡骨草总黄酮的工艺优选[J]. 中国实验方剂学杂志, 2013,19(2):38-40.

LIAO Chun-yan, YANG Xin-lv. Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 38-40.
廖春燕, 杨欣绿. D101型大孔树脂纯化鸡骨草总黄酮的工艺优选[J]. 中国实验方剂学杂志, 2013,19(2):38-40. DOI：

LIAO Chun-yan, YANG Xin-lv. Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 38-40. DOI：

摘要

目的: 优选D101型大孔吸附树脂纯化鸡骨草总黄酮的工艺条件。方法: 以芦丁为对照品

采用紫外比色法测定总黄酮含量

以吸附率和洗脱率为指标

通过单因素试验考察对大孔树脂动态吸附和解吸条件。结果: 最佳工艺条件为上样质量浓度3 g·L-1

上样流速3 BV·h-1

2 BV 80%乙醇为洗脱剂

pH 6.0

洗脱流速3.5 BV·h-1。鸡骨草黄酮纯度由17.95%提高到52.80%。结论: D101型大孔树脂对鸡骨草黄酮类物质有较好的纯化效果

该优选的工艺简单可行。

Abstract

Objective: To optimize purification technology conditions of total flavonoid from Abrus cantoniensis by D101 macroporous resin. Method: With rutin as reference

the content of total flavonoid was determined by UV colorimetry

with adsorption rate and desorption rate as indexes

conditions of absorption and desorption were investigated by single factor test. Result: The best technology conditions were as follows:the concentration of sample solution 3 g· L-1

sample flow rate 3 BV·h-1

with 2 BV 80% ethanol as eluant

pH 6.0

elution flow rate 3.5 BV·h-1.Under these conditions

purity of total flavonoid was increased from 17.95% to 52.80%. Conclusion: D101 macroprous resin had good purification effect for total flavonoid from A. cantoniensis

optimized process was simple and feasible.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

肿节风总黄酮大孔吸附树脂纯化工艺

枳壳有效成分含量与土壤因子的相关性分析

超临界流体色谱分离纯化木香中的木香烃内酯和去氢木香内酯

制川乌炮制废水中生物碱类成分的回收利用

大孔吸附树脂富集纯化三七总皂苷工艺研究