Objective: To investigate the effect of puerarin on parkinson'as rats model induced by 6-hydroxydopamine (6-OHDA). Method: Parkinson's rats model was established

the rats were randomly divided into 5 groups: model group

L-dopa group (40 mg· kg-1)

low-

medium-and high-dosage groups of puerarin (20

40

80 mg· kg-1). The drugs were intragastrically perfused to rats daily for 30 consecutive days. The activity of superoxide dismutase (SOD)

glutathione peroxidase (GSH-Px) as well as the contents of MDA in substantia nigra tissue were tested by biochemical method; the pathological change of nerve cells in substantia nigra was observed by HE staining; then the expressions of induced nitric oxide synthase (iNOS)

cyclic adenosine monophosphate(cAMP)-response-element-binding-protein (CREB) in substantia nigra were using Western blot analysis. Result: The condition of Parkinson's rats model was alleviated via puerarin treatment. Compared to model control group

puerarin significantly elevated the activities of GSH-Px and SOD in substantia nigra tissue of parkinson'as rats

while the MDA contents was reduced (P<0.01); and down-regulated the iNOS protein level in substantia nigra tissue

while the expression of CREB was increased (P<0.01). Conclusion: The findings indicate that puerarin has protective effect on the 6-OHDA-induced PD rats the mechanism may be associated with restraining the oxidative stress and regulations of endogenous iNOS

CREB expressions.