Objective:To study the influence of Bushen Huazhuo Fang (BHF) on improving leptin and insulin resistance in rats with nonalcoholic fatty liver

and to explore its mechanism. Method: Forty-five Wistar rats were randomly divided into blank group

model group

silybin capsules(19 g·kg-1) group and BHF group. The blank group was fed with normal diet

model group

silybin capsules and BHF group were fed with the high fat diet. Silybin capsules and the BHF group were fed with the corresponding drug. After 12 weeks

rats were sacrificed

the rat liver index

Lee’s index

insulin resistance index(IRI)

insulin sensitivity index(ISI) were calculated. Liver chemical parameters

blood lipids

blood glucose

insulin(INS)

leptin(LEP) and tumor necrosis factor and liver Pathological investigation were carried out and evaluated. Result: BHF and silybin could significantly improve the alanine transaminase(ALT)

aspartate transaminase(AST)

LEP (P<0.01); BHF was better than silybin for improving INS

IRI

ISI (P<0.05 or P<0.01)

Silybin had no significant effect on improving INS and IRI. Silybin and BHF groups were better than the model group in fat staining

glycogen staining (P<0.05 or P<0.01). Conclusion: The therapeutic effect of BHF may come from protecting liver

regulating blood lipids

improving LEP and IRI.