LIU Jin-huan, YANG Yu-qin, QING Li-fen, et al. GC Fingerprint of Volatile Constituents of Rhizoma Zingiberis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 153-156.
LIU Jin-huan, YANG Yu-qin, QING Li-fen, et al. GC Fingerprint of Volatile Constituents of Rhizoma Zingiberis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 153-156.DOI:
Objective:To establish chromatographic fingerprint of volatile oil and investigate the variance of Rhizoma Zingiberis collected from different places. Method: First
gather Rhizoma Zingiberis from different places
then smashed and dried out. Second
mark off the different samples and extract the volatile constituents. Third
assay the final samples by GC and correct their fingerprints and make evaluations at last. The chromatography analysis was carried out on an HP-5 capillary column (320 μm×30 m
0.25 μm)
the temperature program was as follows: 60℃ for six minutes
rose to 146℃ at 5℃·min-1
then rose to 165℃ at 2℃·min-1 for five minutes
and then rose to 250℃ at 8℃·min-1
by using helium as the carries gas
the flow rate of 1 mL·min-1
the split ratio 30: 1
sample inlet temperature of 250℃. Result: A total of twenty common peaks identified from the GC fingerprints of volatile oil in Rhizoma Zingiberis.More than eleven samples of representative Rhizoma Zingiberis were chosen for preliminary fingerprint assay. The similarity
repetitiveness
steadiness and precision of these different Rhizoma Zingiberis were obtained. Conclusion: The quality of most of these Rhizoma Zingiberis is stable and controllable
which meet the requirements of the 2005-edition Pharmacopoeia. However
a minority of these Rhizoma Zingiberis has some differences in quality