Objective: To observe the effects of breviscapine on renal cell apoptosis and expression of apoptosis-related proteins in mice with cisplatin-induced nephrotoxicity. Method: Kunming mice were randomly divided into four groups: control group

model group

breviscapine experimental groups by 25 mg·kg-1 or 50 mg·kg-1.Three groups were given a single injection of cis-platinum complexes(CDDP)to establish the model of renal injury(8 mg·kg-1

ip) except the control group

then the mice in two breviscapine experimental groups were given different dose(25

50 mg·kg-1

ig) once a day for seven days.The urine samples were collect for measuring Urine protein(Upr)/Urine creatinine(Ucr) and N-aletyl-beta-D-glucosaminidase(NAG-U).Apoptosis of the renal cells were determined by TUNEL method

also the expression of Bax and Bcl-2 was evaluated by immunohistochemical SP method after the mice were sacrificed. Result: The Upr/Ucr ratio

NAG-U in the model group were higher than those in the control group

but they were significantly lower in two breviscapine groups(P<0.05

P<0.01)

also apoptosis index of the renal cells

the expression of Bax and Bcl-2

the Bax/Bcl-2 ratio in the model group were higher than those in the control group.But

in the breviscapine experimental group of 50 mg·kg-1

apoptosis index was significantly declined(P<0.05

P<0.01)

the expression of Bcl-2 was increased

the expression of Bax was declined

the Bax/Bcl-2 ratio was declined compared with those in model group(P<0.05

P<0.01). Conclusion: The Upr/Ucr ratio and NAG-U might be useful for evaluating renal injury induced by CDDP.Breviscapine could suppress renal cell apoptosis induced by CDDP and protect renal from injury by means of increasing the expression of Bcl-2 and declining the expression of Bax and Bax/Bcl-2 ratio.