Objective: The Cervi Cornu Pantotrichum and Dioscoreae Rhizoma pair(CRP) has been used for kidney-yang deficiency for thousands years.But there is no material about its pharmacological activity.We studied the strengthening yang activity of CRP with kidney-yang deficiency mice model. Method: Forty-eight mice were randomly divided into experimental group

control group

positive control group and model group.The control group and model group mice were given normal saline 0.01 mL·g-1 by gavage

the positive control group mice were given nanbao capsule 1.3 g·kg-1

experimental group mice were given CRP 0.9 g·kg-1.The mice were injected subcutaneously with dexamethasone 0.04 mg except control group mice.After the last administration on the 14th day

several organ indexs of loaded-swimming mice were observed.The antioxidant capacity of CRP was determined by DPPH.The levels of testosterone in Leydig cell was determined by the method of ELISA. Result: The mice with CRP could swim for longer time than model mice

epididymis indexs was also higher(P<0.05).The IC50 of CRP was 114 mg·L-1

and the proliferation rate of spleen cells was 74.13%

51.49%

41.90%

30.41%

21.31% in CRP-treated groups corresponding to CRP concentrations of 1 600

800

400

200

100 mg·L-1

respectively. Conclusion: It is concluded that CRP can be used for reinforcing deficiency.