Objective:To optimize purification technology of total lagnans from Schisandra sphenanthera extract by macroporous resin combined with aluminum oxide. Method: With composite score of impurity removal rate and transfer rate of schisantherin A as index

effect of the amount of resin

ethanol concentration and amount on macroporous resin purification technology of total lignans was investigated by L9(34) orthogonal test;alumina adsorption purifying technology of total lignans from S. sphenanthera was investigated by single factor test.The content of total lignans was determined UV spectrophotometry

HPLC was used to determine the contents of schisantherin A and deoxyschizandrin. Result: Optimum purification conditions of total ligans in S. sphenanthera were as follows:took 2/3-4/3 times the amount of LSA-7 macroporous resin

eluted with 6 BV 85% ethanol

ratio of aluminum oxide and crude drug 1∶1. Conclusion: This optimized purification process was feasible

stable

reasonable and suitable for industrial production.