Objective:To investigate the protective effects of Xinjining (XJN) on acute myocardial ischemia. Method: Acute myocardial ischemia model in rats was established by injecting pituitrin(Pit) into sublingua vein. SD rats were randomly divided into 6 groups: control group

model group

Wenxin granule group (9.0 g·kg-1)

XJN groups (6.0

3.0

1.5 g·kg-1). We monitored electrocardiography(ECG) and recorded the cardiographs of all the groups at the times of Pit pro-injection and post-injection

moreover

compared the change ratio of the T wave height and heart rate (HR)

detected the contents of malonaldehyde (MDA)

superoxide dismutase (SOD)

phosphocreatine kinase (CK) and lactate dehydrogenase (LDH) of blood serum. The acute myocardial ischemia model in SD rats was established by ligating the left anterior descending coronary artery (LAD). The rats were randomly divided into control group

model group

Wenxin granule group (9.0 g·kg-1) and XJN group (3.0 g·kg-1). After 4-day intervention

the acute myocardial ischemia model was established by LAD. After the model established successfully

the rats were given another 3-day intervention

we recorded the hemodynamic indexes

such as

arterial pressure systolic blood pressure(SBP)

diastolic blood presure(DBP)

heart rate(HR)

the left ventricular systolic pressure (LVSP)

the left ventricular end diastolic pressure (LVEDP) and the maximum pressure variation rate of left ventricular in isovolumic phase (±dp/dtmax). Meanwhile

we calculated the data of the left ventricular developed pressure (LVDP). Moreover the activity of SOD

the contents of MDA

CK and LDH in blood serum were determined

and the myocardial infarct size (MIS) was measured after tripheryltetrazolium chloride (TTC) dyeing. Result: XJN (6.0

3.0 g·kg-1) could obviously reduce the changes of T wave at the times of 15 s

2

5 min (P<0.01 or P<0.05).XJN (6.0

3.0

1.5 g·kg-1) could ameliorate HR at the times of 15 s (P<0.05)

30 s (P<0.01).XNJ (6.0

3.0 g·kg-1) could markedly increase the activity of SOD

decrease the contents of LDH

CK

MDA in rats induced by Pit (P<0.01 or P<0.05). XJN (3.0 g·kg-1) could increase the activity of SOD without significance

increase LVSP

LVDP and+dp/dtmax significantly

decrease the contents of MDA

LDH

CK

LVEDP and MIS significantly (P<0.01 or P<0.05) in rats induced by LAD. Conclusion: XJN has significantly protective effects on acute myocardial ischemia in rats.