Objective: Using reversed-phase high performance liquid chromatography (RP-HPLC) method to analyze 6 ingredients of Zhitong prescription one-time. Method: Using Diamond C18 column (4.6 mm×250 mm

5 μm)

the column temperature was 30℃; methanol acetonitrile 0.1% ammonia water as the mobile phase

1 mL·min-1 gradient elution; detection wavelength was 230 nm (paeoniflorin)

235 nm (the aconitine hypaconitine)

280 nm (tetrahydropalmatine)

280 nm (protopine) and 235 nm (mesaconitine). Result: The peak area and concentration of the six components showed good linearity (r>0.999 4) the paeoniflorin aconitine hypaconitine

tetrahydropalmatine protopine

new aconitine the recoveries were 97.2% (RSD 1.4%)

96.5% (RSD 1.2%)

102.5% (RSD 0.7%)

96.8%(RSD 0.7%)

100.8%(RSD 1.1%)

98.5%(RSD 1.1%). Conclusion: By using RP-HPLC simultaneous determination of the active ingredients of the the analgesic prescription of several simple

we can provide a basis for the quality control of the preparation of Zhitong prescription.