微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较

方婧; 杨洪军; 付梅红; 安琪; 张贝贝; 王祝举; 杨岚; 张东

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微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较

更新时间：2024-01-04

- 微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较
- Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method
- 中国实验方剂学杂志 2012年18卷第24期页码：94-96
- 作者机构：
- 作者简介：
- 基金信息：
  
  "重大新药创制"科技重大专项(2009ZX09308-003);"国家科技支撑计划子课题"(2006BAI09B07-02)
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- 纸质出版日期：2012
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方婧, 杨洪军, 付梅红, 等. 微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较[J]. 中国实验方剂学杂志, 2012,18(24):94-96.

FANG Jing, YANG Hong-jun, FU Mei-hong, et al. Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 94-96.
方婧, 杨洪军, 付梅红, 等. 微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较[J]. 中国实验方剂学杂志, 2012,18(24):94-96. DOI：

FANG Jing, YANG Hong-jun, FU Mei-hong, et al. Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 94-96. DOI：

摘要

目的: 建立微波协助提取法提取野菊花中有效成分蒙花苷含量测定方法

比较微波协助提取法与药典法提取蒙花苷优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相甲醇-水-冰醋酸(26∶23∶1)

检测波长334 nm

柱温30 ℃

流速1 mL·min-1。结果: 微波提取时间15 min

提取温度100 ℃

提取溶剂50%乙醇。蒙花苷在0.04~1.2 μg呈良好线性关系(r=0.999 9)

平均回收率100.13%(n=6)。结论: 微波提取法较药典法提取蒙花苷更简便快速、准确

为一种符合环保绿色理念的含量测定方法

可用于检测野菊花饮片中蒙花苷含量。

Abstract

Objective:To establish a microwave-assisted extraction method for the extraction of the linarin from Chrysanthemun indicum. Comparison of the determination of linarin from C. indicum between microwave-assisted extraction and Chinese pharmacopeia method was made. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm

5 μm)was used. The mobile phase cousisted of methanol-water-acetic acid (26∶23∶1).The temperature of column was kept at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wave-length was at 334 nm. Result: Microwave extractive time was 15 min

the temperature was 100 ℃

the extraction solvent was 50% ethanol. The calibration curve was in good linearity within the range from 0.04 to 1.2 μg(r=0.999 9). The average recovery was 100.13%(n=6). Conclusion: The method is simple

rapid

as well as precise and reliable

and can be used for the determination of linarin from C. indicum.

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