Objective: To study the anti-metastasis effect and mechanism of Liujun Qutan Jiedu decoction and its functional groups in metastasis of C57 BL/6J mice Lewis lung cancer. Method: The Lewis lung metastasis model was established in C57 BL/6J mice

randomly divided into 6 groups: saline model group

Tegafur positive control group (78 mg·kg-1)

Liujun Qutan Jiedu decoction (21 g·kg-1)

Liujun Qutan decoction (11 g·kg-1)

Jiedu decoction (10 g·kg-1)and Qutan decoction (5 g·kg-1).After oral administration for 16 days

the tumor was stripped and weighed to calculate the inhibition rate.The metastatic tumor foci on lung surface in mice were counted to calculate the rate of cancerometastasis and anti-metastasis.Expression of CD44 in tumor cells was measured by immunohistochemistry and image analysis system

the concentrations of serum hyaluronic acid(HA) and collagen Ⅳ(Col Ⅳ) were detected by radioimmunoassay. Result: Liujun Qutan Jiedu decoction and its functional groups could inhibit the metastasis of Lewis lung carcinoma.The metastatic foci occurrence rates of treatment groups on lung surface were lower than model group.The effect of Liujun Qutan Jiedu decoction was the best.All treatment groups can inhibit the expression of CD44 to some degree.Compared with the model group

Liujun Qutan Jiedu decoction had a significant difference(P<0.05)

Liujun Qutan decoction and Qutan decoction had a more significant difference(P<0.01);All four treatment groups could reduce the concentrations of serum HA and Col Ⅳ

however

the effect of Liujun Qutan decoction was the best

the effect of Liujun Qutan Jiedu decoction and Qutan decoction were the second(P<0.01)

and the function of Jiedu decoction was relatively weak. Conclusion: Liujun Qutan Jiedu decoction and its functional groups all have anti-metastasis activities on Lewis lung metastasis model in C57BL/6J mice

and the underlying mechanisms were possibly related to inhibiting the breaking of extracellular matrix(ECM) caused by tumor cells

reducing the concentrations of serum HA and Col Ⅳ.At the same time

the mechanism also related to inhibiting tumor cells moving

attacking and transferring by reducing the expression of CD44 and its adhesion to ECM.