Objective:To study on dynamic variation law of bufalin

cinobufagin and recibufogenin from fresh pulp of Bufo bufo gargarizans by placing at different temperatures

in order to guide processing of B. bufo gargarizans. Method: The content of bufalin

cinobufagin and recibufogenin in fresh pulp of B. bufo gargarizans were determined by HPLC

which was respectively stored at room temperature

refrigerated and frozen conditions. Result: At room temperature and refrigerated conditions

the content of these three components increased with extension of storage time

and they elevated greatly and proporation of them changed obviously in the first 24 hours. Conclusion: Before fresh pulp of B. bufo gargarizans was processed to B. bufo gargarizans

it should place at room temperature more than 24 hours

and then to do dry processing.