Objective:To optimize extraction technology of total flavonoids in pinecone of Pinus yunnanensis. Method: Total flavonoids in pinecone of P. yunnanensis were extracted by ultrasonic assisted extraction method

with extraction rate of total flavonoids as index

orthogonal test was applied to investigate influence of four factors (including ratio of material-liquid

ultrasonic extraction temperature

ethanol concentration and extraction time) on extraction technology of total flavonoids from pinecone of P. yunnanensis. Result: Optimum extraction technology was as following:ultrasonic assisted extracted 30 min with 30 times the amount of 60% ethanol at 60℃

the content of total flavonoids in pinecone of P. yunnanensis was 3.23%. Conclusion: Optimized extraction technology was simple and stable with high extraction rate of total flavonoids.