Objective:To observe the influence of energy charge and the expression of ciliary neurotrophic factor mRNA(CNTF mRNA) by Fuzi Lizhong decoction in rats with spleen yang deficiency syndrome. Method: Animals were divided into the control goup

the model group and the Fuzi Lizhong decoction group. The model group was insised brown adipose tissue then fed with high fat diet under 19℃ circumstance

which lasted for 3 weeks.The Fuzi Lizhong decoction group was ig 5 mL·kg-1 Fuzi Lizhong decoction based on the model group once a day

for 4 weeks. Testing energy charge of skeletal muscle and the expression of CNTF mRNA were assayed by the method of reverse transcription-polymerase chain reaction(RT-PCR). Result: Compared with the control group(0.042±0.018)

energy charge decreased in the model group (0.032±0.014)and the Fuzi Lizhong decoction group (0.024±0.012 )( P<0.05 or P<0.01). Compared with the model group

energy charge decreased significantly (P<0.05). Compared with the control group(0.862±0.072)

the expression of CNTF mRNA in the model group (0.426±0.056) decreased(P<0.01). Compared with the model group

the expression of CNTF mRNA in the Fuzi Lizhong decoction group (0.838±0.068)increased (P<0.01). Conclusion: It is suggested that Fuzi Lizhong decoction can regulate the expression of CNTF mRNA

which indicated an effect on nutrition and energy metabolism of skeletal muscle by up-regulating the expression of CNTF mRNA and increasing the content of CNTF.