MA Wen-Fang, DENG Hui-lian, CAI Yi, et al. Research on the Determination of Total Flavonoids Content and Colour Reaction System in[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 112-115.
MA Wen-Fang, DENG Hui-lian, CAI Yi, et al. Research on the Determination of Total Flavonoids Content and Colour Reaction System in[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 112-115.DOI:
Objective:To establish a quantitative method for the determination of total flavonoids in Scoparia dulcis by UV-VIS spectrophotometry. Method: The effects of species
concentration
and dosage of chromogenic reagent were studied
and NaNO2-Al(NO3)3-NaOH colour reaction system was used for content determination of total flavonoids in Scoparia dulcis. Result: Using rutin as comparison sample
the detection wave length was set at 506 nm. There was good linear relationship during 4.8-96.2 mg·L-1 (r=0.999 3). The average recovery rate was 103.71% (RSD 2.68%
n=6). Conclusion: The method is simple
rapid
accurate and can be used for the quantitative method of S. dulcis.