HAN Qiu-jun, BI Wei, Wang Wei, et al. Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 86-88.
HAN Qiu-jun, BI Wei, Wang Wei, et al. Comparative on Peptide Content in Pre-and-Post-Processed Trionycis Carapax[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 86-88.DOI:
Objective:A rapid method was established for quantitative determination of peptide based on biuret reaction-enzyme-linked immunosorbent detector
and peptide content in the same geographical origin of pre-and-post-processed Trionycis Carapax was analyzed. Method: The developer was 0.02 g·mL-1 sodium potassium tartrate solution (50 μL)
5% NaOH (20 μL)
1% CuSO4·5H2O (50 μL). The wavelength of detection was at 580 nm. Result: The calibration curves were linear in the ranges of 0.00-5.00 g·L-1 for septa-peptide from Trionycis Carapax. Peptide content in vinegar-processed samples was 6.99%
while the same content in crud samples was 1.04%. Conclusion: Peptide content in vinegar-processed samples was significantly higher than the one in crud samples. The content of Trionycis Carapax is increased greatly after vinegar by decoction.