兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响

张纨; 王志坤; 董林林; 李博林; 吴云楚; 刘启泉

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兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响

更新时间：2024-01-04

- 兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响
- Effect of Lanyin Fengyang Huazhuo Jiedu Prescription on Immune Regulation Mechanism in Rats with Ulcerative Colitis
- 中国实验方剂学杂志 2013年19卷第1期页码：236-239
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省中医药管理局科研计划项目(2009015);河北省中医药管理局溃疡性结肠炎浊毒证研究室课题
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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张纨, 王志坤, 董林林, 等. 兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响[J]. 中国实验方剂学杂志, 2013,19(1):236-239.

ZHANG Wan, WANG Zhi-kun, DONG Lin-lin, et al. Effect of Lanyin Fengyang Huazhuo Jiedu Prescription on Immune Regulation Mechanism in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 236-239.
张纨, 王志坤, 董林林, 等. 兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响[J]. 中国实验方剂学杂志, 2013,19(1):236-239. DOI：

ZHANG Wan, WANG Zhi-kun, DONG Lin-lin, et al. Effect of Lanyin Fengyang Huazhuo Jiedu Prescription on Immune Regulation Mechanism in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 236-239. DOI：

摘要

目的: 观察兰茵凤扬化浊解毒方对溃疡性结肠炎(UC)大鼠CD4-T淋巴细胞(CD4+)/CD29-T淋巴细胞(CD29+)、白细胞介素-8(IL-8)及白细胞介素-10(IL-10)表达的影响

以探讨其作用机制。方法: 将60只雄性Wistar大鼠随机分为5组

每组12只

分别为正常组、模型组、兰茵凤扬化浊解毒方高剂量组、低剂量组(20.0

5.0 g·kg-1)、柳氮磺胺吡啶对照组(SPAP 0.3 g·kg-1组)。采用2

6-三硝基苯磺酸(TNBS)/乙醇法诱导溃疡性结肠炎模型

造模成功后分别ig给予相应的药物

连续14 d

观察各组大鼠一般情况以及疾病活动指数(DAI)评分、组织病理学、CD4+CD29+

IL-8

IL-10变化情况。结果: DAI评分:与正常组比较

其余各组均升高;与模型组比较

兰茵凤扬高、低剂量组均减低

结果均有统计学意义(P<0.05)。CD4+

CD29+:与模型组相比

对照组、中药高、低剂量组均降低

结果有统计学意义(P<0.05)。IL-8: 与模型组相比

对照组、中药高、低剂量组均减低(P<0.05)

而中药低剂量组与对照组比较无差异。IL-10: 与模型组相比

中药高、低剂量组、对照组均升高(P<0.05)。结论: 兰茵凤扬化浊解毒方可上调抑炎症因子、下调促炎症因子和抑制CD4+/CD29+的表达。

Abstract

Objective:To observe the effect of Lanyin Fengyang Huazhuo Jiedu prescription on expressions of CD4+/CD29+

IL-8 and IL-10 in rats with ulcerative colitis. Method: Sixty healthy male Wistar rats were randomly divided into 5 groups: the normal group(NG)

model control group (MG)

high-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (HDG

20.0 g·kg-1)

low-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (LDG

5.0 g·kg-1) and the sulfasalazine group(SPAP 0.3 g·kg-1)

12 rats in every group. Trinitrobenzenesu l-fonicacid/ethanol(TNBs/ethanol) was used to induce ulceratvie colitis. After successful modeling

continuous treatment lasted 14 days. The rat's general state

disease activity index(DAI) scores

colonic pathological morphology change of T-lymphocyte cytokines IL-8 and IL-10 were observed. Result: DAI scores: compared with NG

the scores of other groups were elevated. compared with the MG

the scores of HDG and LDG were reduced. The result showed statistical significance (P<0.05). CD4+

CD29+: compared with the MG

the levels of NG

HDG and LDG were reduced. The result showed statistical significance (P<0.05). IL-8: compared with the MG

the levels of NG

HDG and LDG were reduced (P<0.05). There was no difference between the levels of LDG and SG. IL-10: compared with the MG

the levels of SPAP

HDG and LDG were elevated(P<0.05). Conclusion: Lanyin Fengyang Huazhuo Jiedu decoction can raise the suppression of inflammatory cytokines

reduce promoting the inflammatory factor and suppressioned of CD4+/CD29+expression

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