穿龙薯蓣HPLC指纹图谱及模式识别

尹海波; 康廷国; 张囡; 杨小乐

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穿龙薯蓣HPLC指纹图谱及模式识别

更新时间：2024-01-04

- 穿龙薯蓣HPLC指纹图谱及模式识别
- HPLC Fingerprint Analysis and Pattern Recognition of
- 中国实验方剂学杂志 2012年18卷第22期页码：145-148
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省教育厅项目(2009A498);校优秀青年药学人才基金(yxrc0904)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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尹海波, 康廷国, 张囡, 等. 穿龙薯蓣HPLC指纹图谱及模式识别[J]. 中国实验方剂学杂志, 2012,18(22):145-148.

YIN Hai-bo, KANG Ting-guo, ZHANG Nan, et al. HPLC Fingerprint Analysis and Pattern Recognition of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 145-148.
尹海波, 康廷国, 张囡, 等. 穿龙薯蓣HPLC指纹图谱及模式识别[J]. 中国实验方剂学杂志, 2012,18(22):145-148. DOI：

YIN Hai-bo, KANG Ting-guo, ZHANG Nan, et al. HPLC Fingerprint Analysis and Pattern Recognition of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 145-148. DOI：

摘要

目的: 建立穿龙薯蓣药材HPLC指纹图谱。方法: Agilent TC-C18(4.6 mm×250 mm

5 μm)色谱柱;流动相乙腈(A)-水(B)梯度洗脱

检测波长203 nm

流速1.0 mL·min-1。收集了不同产地野生和栽培的28批样品进行测定

采用主成分分析筛选出12批样品

聚类分析结果与主成分分析结果相似

剔除1个距离较远的样品

最终筛选11批样品建立指纹图谱共有模式。结果: 确立了14个共有峰

方法学考察结果良好

11批样品的相似度均>0.866。结论: 该方法可用于穿龙薯蓣质量控制及综合评价。

Abstract

Objective:To establish Dioscorea nipponica HPLC fingerprint. Method: The separation was performed on a Agilent TC-C18(4.6 mm×250 mm

5 μm) column

with acetonitrile-water as mobile phase for gradient elution; the UV detector was set at 203 nm; the flow rate was 1.0 mL·min-1. There were 28 samples of wild and cultivated from various places to be analyzed. There were 12 samples selected by principal component analysis (PCA)

the result of cluster analysis was similar with PCA

except one sample which had been eliminated. 11 samples were selected to establish the common pattern of the fingerprint. Result: 14 marked common peaks were established

inspection results of the method were good

the similarities of 11 samples were more than 0.866. Conclusion: The method provides an academic reference for the quality control of Dioscorea nipponica.

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