Objective: To study the influence of the extract of Polygoni Multiflori Radix on follicle growth and hair growth of C57BL/6J. Method: The model of organ culture of mouse vibrissa follicle in vitro was used and the time and velocity of hair growth were recorded to investigate the influence of follicle growth. The experiment had 9 groups: blank control group

positive control group (Zhangguang 101)

different content of the extent for 75

150

300

600

1200

2 500

5 000 μg·L-1

24 shadow mask

2 folliculus pili per hole and cultivating 12 d. Treated in vitro on hairless mice skin was used to investigate the effects of promoting hair growth with different dose of Polygoni Multiflori Radix. This experiment had 5 groups: positive control group (Zhangguang 101)

blank control group

the extract of Polygoni Multiflori Radix high dose group(2 g·mL-1)

medium dose group (1 g·mL-1)

low dose group (0.5 g·mL-1). Result: The experiment of organ culture of mouse vibrissa follicle in vitro showed that the extract of Polygoni Multiflori Radix could accelerate follicle growth in the early

intermediate and terminal phases and prolong the hair growth time with the dose of 300

600

1 200 μg·L-1 in a dose-dependent manner and there were significant differences in comparison with normal group. When the content was 300 μg·L-1

the velocity of hair growth were(0.236±0.061)

(0.427±0.078)

(0.325±0.054)mm·d-1 on the second

sixth and twelve day

the time of hair growth was (9.60±1.43)d

the differences were significant between the test groups with the blank control and positive control group(P<0.01). When the content was 600 μg·L-1

the velocity of hair growth were(0.313±0.044)

(0.522±0.084) mm·d-1 on the second and sixth day

the differences were significant between the test groups with the blank control group(P<0.01)and between the test groups with the positive control group (P<0.05)

the velocity of hair growth was(0.439±0.064)mm·d-1 on the twelve day

the time of hair growth was (11.00±1.15)d

the differences were significant between the test groups with the blank control group (P<0.01). When the content was 1 200 μg·L-1

the velocity of hair growth were (0.406±0.053)

(0.642±0.067)

(0.475±0.036)mm·d-1 on the second

sixth and twelve day

the time of hair growth was (12.40±1.43) d

the differences were significant between the test groups with the blank control group(P<0.01).The experiment of pharmacodynamics in vivo showed the extract of Polygoni Multiflori Radix can make hair grow from anestrum to growing period with significant difference. High dose group can accelerate the production of melanin in skin than middle and low dose group. The time of high dose group were (9.06±0.43) d when the skin became pale from pink

(14.68±1.19) d when hair came out and (26.86±2.02) d when hair was overgrown

the differences were significant between the test groups with the blank control group(P<0.01). In the later period of hair growth

the dependence on its concentration is not significant. Conclusion: The extract of Polygoni Multiflori Radix can lead hair growth of C57BL/6J mice from anestrum to growing period

the result of experimental in vivo and in vitro is coincident.