HPLC测定大血藤药材中绿原酸的含量

丁越; 张永; 丁文平; 张彤; 陶建生; 荣蓉

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HPLC测定大血藤药材中绿原酸的含量

更新时间：2024-01-04

- HPLC测定大血藤药材中绿原酸的含量
- Determination of Chlorogenic Acid in by High Performance Liquid Chromatography
- 中国实验方剂学杂志 2013年19卷第4期页码：99-102
- 作者机构：
- 作者简介：
- 基金信息：
  
  上海市科委资助项目(09dZ1975500);上海市人才发展基金项目(20111015);上海市教委基金项目(10SG40,J50302)
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- 纸质出版日期：2013
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丁越, 张永, 丁文平, 等. HPLC测定大血藤药材中绿原酸的含量[J]. 中国实验方剂学杂志, 2013,19(4):99-102.

DING Yue, ZHANG Yong, DING Wen-ping, et al. Determination of Chlorogenic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 99-102.
丁越, 张永, 丁文平, 等. HPLC测定大血藤药材中绿原酸的含量[J]. 中国实验方剂学杂志, 2013,19(4):99-102. DOI：

DING Yue, ZHANG Yong, DING Wen-ping, et al. Determination of Chlorogenic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 99-102. DOI：

摘要

目的: 建立大血藤药材中绿原酸的含量测定方法。方法: 采用高效液相色谱法

Diamonsil ODS-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-4%磷酸水(11:89)

流速1.0 mL·min-1

检测波长327 nm

柱温25℃。结果: 绿原酸在0.000 102~0.010 2 mg 线性关系良好。加样回收率为102.4%

RSD 1.7%(n=6)。结论: 采用HPLC测定大血藤药材中绿原酸的含量

样品处理方法便捷

测定方法简单

结果准确可靠

可用于大血藤饮片、大血藤制剂的质量控制。

Abstract

Objective: To establish the determination method of chlorogenic acid in Sargentodoxa cuneata. Method: HPLC was used with Diamonsil ODS-C18 column (4.6 mm×250 mm

5 μm) as chromatographic column and acetonitrile-4% phosphoric acid solution (11:89) as mobile phase. The flow rate was 1.0 mL·min-1; the detection wavelength was set at 327 nm and the column temperature was kept at 25℃. Result: The linear range of chlorogenic acid was 0.000 102-0.010 2 mg. The average recovery was 102.57%

RSD 0.83% (n=6). Conclusion: The method of sample processing was simple and the HPLC method was accurate and reliable. It can be used for the quality control of S. cuneata and its preparation.

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