Objective:To compare the content of volatile oil from Acorus tatarinowii in three areas of Anhui

Jiangxi

Hu’nan

and optimize β-CD different inclusion technology of volatile oil. Method: Volatile oil from A. tatarinowii was extracted by steam distillation method

and extraction volume of volatile oil in different time was compared.Taking inclusion rate of volatile oil as index

orthogonal test was used to optimize inclusion technology of saturated aqueous solution

ultrasound and sealing temperature control methods

then to determine optimum β-CD inclusion method and technology. Result: Yield of volatile oil from A. tatarinowii in Jiangxi

Anhui

Hu’nan were 1.15%

1.39%

1.65%

respectively.β-CD inclusion rate of saturated aqueous solution method

ultrasonic method and sealing temperature control technology were 83%

61%

56%

respectively.Optimum inclusion method was saturated aqueous solution method

its optimal inclusion technology was as following:volatile oil-β-CD-water 1∶8∶60

stirred 2 h at 50 ℃. Conclusion: Content of volatile oil from A. tatarinowii in Hu’nan was the highest in these three areas.Optimized inclusion technology was reasonable and economic with high inclusion rate.