Objective: To optimize separation and purification technology of total flavonoids from Citrus aurantium by AB-8 macroporous resin. Method: Adsorption property of seven different kinds of macroporous resins for total flavonoids from C. aurantium was compared by static adsorption test

in order to select optimum macroporous resin;Single factor test was used to investigate adsorption and elution conditions of macroporous resin. Result: AB-8 macroporous resin was the most suitable one for purification of total flavonoids in C. aurantium;Its optimum separation and purification parameters were as followings:the sample concentration 0.408 g·L-1

pH of 4

sample flow rate 2 BV·h-1

eluted with 6 BV 75% ethanol at 1 BV·h-1;Under this condition

purity of total flavonoids would be increased from 14.03% to 42.54%. Conclusion: Optimized separation and purification technology was stable and feasible

it could be extended to industrial production.