Objective: To develop a capillary gas chromatographic method for detection and determination of 19 organochlorine pesticide residues in Chinese herbal medicines. Method: Sample was extracted with ethyl acetate using an ultrasonic bath

and cleaned up with solid phase extraction cartridges

Carbon-GCB-Florisil in series. Analysis was carried out on a fused silica capillary column (DB-35

0.53 mm×30 m

0.25 μm film). The temperature program was as follows:initial temperature at 80℃

the 40℃·min-1 to 200℃

then 5℃·min-1 to 300℃

and kept for 2 min. The injection port temperature was set at 300℃. The injection mode was splitless. The temperature of electron capture detector was set at 300℃. The content was calculated with external standard method. Result: The average recoveries of 19 organochlorine pesticides were from 80.06% to 124.0%

and RSD values were from 3.5% to 10.7%. The content of the organochlorine pesticides in five Chinese herbal medicines

Uncariae Ramulus Cununcis

Chrysanthemi Flos

Sophorae Flavescentis Radix

Corydalis Rhizoma and Curcumae Radix

was determined. Conclusion: The method was simple

fast

accurate

and can be used for detection and determination of organochlorine pesticide residues in Chinese herbal medicines.