Objective:To prepare triptolide- folic acid chitosan nanoparticle with targeting

and study on its in vitro release property. Method: With particle size and PDI as indexes

influence of solution pH

reaction temperature

proportion and mass concentration of chitosan and sodium polyphosphate on preparation technology of chitosan nanoparticle were investigated by single factor test and orthogonal design;Optimum preparation technology was selected by coupling ratio of triptolide and chitosan

amino reaction of triptolide

folic acid active ester and chitosan

triptolide-folic acid chitosan nanoparticle was prepared by ionic crosslinking method.In vitro release property was examined by HPLC. Result: Optimum preparation technology was as following:reaction temperature 25 ℃

solution pH of 3.5

chitosan-sodium polyphosphate 3∶1

the mass concentration of chitosan and sodium polyphosphate of all 0.3%.Average particle size of prepared nanoparticle was about 170 nm

PDI was about 0.21.Release rate of drug-loading nanoparticle reached equilibrium after 4 h

the maximum release rate was about 68%. Conclusion: Quality of triptolide-folic acid chitosan nanoparticle was stable and reliable

which was prepared by optimized preparation technology

and optimized technology was simple.