HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量

王婴; 王岩; 郭子扬; 钟志良; 叶国强

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HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量

更新时间：2024-01-04

- HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量
- Content Determination of Ginsenosides and Vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii by HPLC
- 中国实验方剂学杂志 2013年19卷第1期页码：122-125
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省科技计划项目(粤科规划字[2012]145号)
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  中图分类号：
- 纸质出版日期：2013
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王婴, 王岩, 郭子扬, 等. HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量[J]. 中国实验方剂学杂志, 2013,19(1):122-125.

WANG Ying, WANG Yan, GUO Zi-yang, et al. Content Determination of Ginsenosides and Vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 122-125.
王婴, 王岩, 郭子扬, 等. HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量[J]. 中国实验方剂学杂志, 2013,19(1):122-125. DOI：

WANG Ying, WANG Yan, GUO Zi-yang, et al. Content Determination of Ginsenosides and Vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 122-125. DOI：

摘要

目的: 建立复方西洋参泡腾片中人参皂苷Rg1、Re、Rb1及维生素C的含量测定方法。方法: 采用高效液相色谱法

色谱柱为Diamonsil C18柱(4.6 mm×250 mm

5 μm)

人参皂苷及维生素C的流动相分别为乙腈-0.1%磷酸溶液(梯度洗脱)和甲醇-0.1%磷酸溶液(3:97)

检测波长203 nm和254 nm。结果: 人参皂苷Rg1线性范围0.050 4~1.26 μg

平均加样回收率98.59%;人参皂苷Re线性范围0.336~8.4 μg

平均加样回收率99.11%;人参皂苷Rb1线性范围1.052~26.3 μg

平均加样回收率98.12%;维生素C线性范围0.806 4~20.16 μg

平均加样回收率97.50%。结论: 建立的方法简便、重复性好、准确度高

可用于该产品的质量控制。

Abstract

Objective:To establish the content determination method for ginsenoside Rg1

ginsenoside Re

ginsenoside Rb1 and vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii. Method: HPLC method was adopted. The chromatographic column was Diamonsil C18 (4.6 mm×250 mm

5 μm) column. The mobile phases were acetonitrile-0.1% phosphate solution (gradient elution) and methanol-0.1% phosphate solution (3:97) respectively. The detection wavelengths were set at 203 nm and 254 nm. Result: The concentration have good linear correlations within the range of 0.050 4-1.26 μg for notoginsenoside Rg1

0.336-8.4 μg for ginsenoside Re

1.052-26.3 μg for notoginsenoside Rb1 and 0.806 4-20.16 μg for vitamin C. The average recovery rates were 98.59%

99.11%

98.12% and 97.50% respectively. Conclusion: The method is simple

reproducible and accurate

and applicable for the quality control of the product.

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