HPLC同时测定小蜡树叶中槲皮素与山奈素的含量

覃兰芳; 胡琦敏; 赖茂祥; 屈信成; 黄云峰; 梁冰

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HPLC同时测定小蜡树叶中槲皮素与山奈素的含量

更新时间：2024-09-23

- HPLC同时测定小蜡树叶中槲皮素与山奈素的含量
- Simultaneous Determination of Quercetin and Kaempferol in Leaf of by HPLC
- 中国实验方剂学杂志 2013年19卷第3期页码：107-109
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西壮药质量标准研究项目(MZY2010038)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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覃兰芳, 胡琦敏, 赖茂祥, 等. HPLC同时测定小蜡树叶中槲皮素与山奈素的含量[J]. 中国实验方剂学杂志, 2013,19(3):107-109.

QIN Lan-fang, HU Qi-ming, LAI Mao-xiang, et al. Simultaneous Determination of Quercetin and Kaempferol in Leaf of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 107-109.
覃兰芳, 胡琦敏, 赖茂祥, 等. HPLC同时测定小蜡树叶中槲皮素与山奈素的含量[J]. 中国实验方剂学杂志, 2013,19(3):107-109. DOI：

QIN Lan-fang, HU Qi-ming, LAI Mao-xiang, et al. Simultaneous Determination of Quercetin and Kaempferol in Leaf of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 107-109. DOI：

摘要

目的: 建立同时测定小蜡树叶中槲皮素与山奈素含量的分析方法。方法: 采用HPLC

色谱柱为Ultimate C18柱 (4.6 mm×250 mm

5 μm)

以甲醇-0.4%磷酸溶液(55∶45) 为流动相

流速1.0 mL·min-1

检测波长360 nm。结果: 小蜡树叶中槲皮素与山奈素的线性范围分别为0.058~0.525 μg和0.203~0.712 μg

平均加样回收率分别为103.0%

107.6%(n=6)。结论: 该方法准确可靠

结果稳定

重复性好

为评价小蜡树叶的质量提供依据。

Abstract

Objective:To establish an analytical method on simultaneous determination of quercetin and kaempferol in leaf of Ligustrum sinense. Method: The analysis was carried out on a column eluted with a mobile phase of methanol-0.4% phosphoric acid solution (55∶45). The flow rate was 1.0 mL·min-1; the detection wavelength was set at 360 nm. Result: The calibration curves of quercetin and kaempferol were linear in the ranges of 0.058-0.525 μg and 0.203-0.712 μg

and the average recoveries were 103.0% and 107.6% (n=6)

respectively. Conclusion: The method is accurate

reliable and repeatable with stable result. It can be used as a method of quantitative determination of quercetin and kaempferol in the leaf of L. sinense

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