Objective:To optimize extraction and separation technology of total saponins from aboveground part of Cimicifuga dahurica. Method: With the content of total saponins as index

segmented extraction method was used to extract active ingredients from aboveground part of C. dahurica with different reagents

solvent was recovered by rotary evaporation

then n-butanol extract was further separated by macroporous resin column

and quantitative detection was determined by UV spectrophotometer. Result: After n-butanol extraction substances over macroporous adsorption resin

purity of total saponins could reach more than 50%. Conclusion: After aboveground part of C. dahurica was further separated by extracting

active ingredients were segmented according to polarity

it could facilitate for late further separation and purification of total saponins from aboveground part of C. dahurica

at the same time

high purity of total saponins could obtain.