Objective:To study the effect of Shenfu injection on endogenous subs-tances in plasma of acute cardiogenic shock rats. Method: Thirty-two Sprague-Dawley rats were randomly divided into control group

model group

positive group

administration group

with ten rats in each group. Each group was made by blocking left coronary artery except the control group

the positive group was administrated with Dopamine injection. the administration group was administrated with Shenfu injection.The control group and model group were only administrated with normal saline. Fourty-five minutes later

plasmawas collected from cardiac apex. Liquid chromatography-mass spectrometry was used to analyze the endogenous compounds

principal component analysis (PCA) was used to observe the difference between the four groups

and orthogonal signal correction-partial least square was used to discriminate analysis (OSC-PLS-DA) of biomarkers. Result: In the scatter plot of PCA

there was no samples coincidence among the four group. The further analysis on loading plot of OSC-PLS-DA indicated that creatine

3-hydroxydodecanoic acid

arachidonic acid

lysophospholipid.etc have changed in the different groups. Conclusion: Shenfu injection has important effect on the endougenous substances in the acute cardiogenic shock rats. Creatine

spermine

3-hydroxydodecanoicacid

arachidonicacid

lysoph-ospholipid may be the potential biomarkers.