Objective: To study influence of different extraction technology on chemical compositions changes from addition and subtraction prescription of Huoluo Xiaoling Dan(HLXL) by HPLC fingerprint. Method: With swertiamarin

paeoniflorin

cryptotanshinone

isoimperatorin and other seven characteristic fingerprint peaks as index

which were determined by HPLC

with YMC-Pack ODS-A column

methonal-water(gradient elution) as mobile phase

detection wavelength 210 nm

flow rate 0.8 mL·min-1

column temperature 30 ℃.Effects of different solvents(water

ethanol

acetone)

extract models(ultrasound

refluxing

macerating) and mixed-extract and separated-extract on relative content of each characteristic peaks were systematically investigated. Result: Different technology extracts had significant difference on relative peak area of eleven evaluating indexes.Kinds of solvent and extraction types and order of mixing had a great influence upon extraction efficiency of chemical compositions from HLXL. Conclusion: This method was reproducible

precise

stable and could be applied to study on fingerprint of HLXL with different extraction technology.