Objective: To determine 14 active compositions alignment (ACA) in Gengen Qinlian Decoction so as to provide technology and ideas for the establishment of quality standard in accordance with its therapeutic. Method: Gengen Qinlian Decoction was prepared by traditional method

the ACA were determined by high performance liquid chromatography (HPLC). Result: The 14 ACA in Gengen Qinlian Decoction were puerarin

daidzin

daidzein

baicalin

wogonoside

baicalein

wogonin

berberine hydrochloride

palmatine hydrochloride

jatrorrhizine hydrochloride

epiberberine hydrochloride

coptisine hydrochloride

ammonium glycyrrhizinate

liquiritin. The content of ACA (%) were 1.673 7%

0.153 3%

0.047 3%

0.448 0%

0.091 6%

0.012 3%

0.006 7%

0.095 5%

0.034 3%

0.043 8%

0.036 0%

0.048 3%

0.110 3%

0.158 0%. Conclusion: The HPLC method used is rapid and simple

and the ACA in Gengen Qinlian Decoction can be used as the scientific indicator of quality control.