Objective:To establish a method for the determination of volatile oil and the determination of eucalyptol in volatile oil as an indicator of Magnoliae Flos (Magnolia biondii) by GC. Method: The content of volatile oil of the mainstream 10 cultivars of M. biondii was determined by determination of volatile oil from the 2010 edition of Chinese Pharmacopoeia

and the content of eucalyptol of the mainstream 10 cultivars was determined by GC. The GC-MS conditions with HP-5MS column (0.25 μm×0.32 mm×30 m) was used

FID detector

the hold air consisted of high purity He

temperature programmed: initial temperature 80℃

retain 6 min

rise temperature to 160℃ at the rate of 20℃·min-1

retain 2 min; rise temperature to 220℃ at the rate of 60℃·min-1

retain 5 min; inlet temperature 240℃; detector temperature 240℃. Result: The content of volatile oil: 3.24%

4.64%

6.12%

3.64%

5.48%

6.98%

3.64%

3.24%

7.68%

2.88%. Content of eucalyptol determined by GC: the linear range was 1.1-10.45 μg(r=0.999 8); the average recovery was 100.02%

RSD 1.12%. The content of volatile oil: 2.076%

4.212%

3.689%

0.1041%

1.590%

3.523%

2.930%

1.940%

4.520%

1.494%. Conclusion: According to the volatile oil content and the content of eucalyptol determined by GC

there were differences in different cultivars of M. biondii

which provided scientific basis for the quality assessment and quality control standards of different cultivated species of M. biondii.