Objective: To investigate the effect of Wenshen Tongluo Zhitong granule on osteoporosis in rat ovariectomized model

to explore its mechanism. Method: Ninety female rats were randomly divided into control group

model group

low-dose

medium-dose

high-dose Wenshen Tongluo Zhitong granule groups and nyestrid(CEE3) group. Animal models of osteoporosis was induced with the ovariectomized method

and intragastric administration was performed.The bone mineral density(BMD)

calcitonin(CT)

bone Gla protein (BGP) and estradiol(E2) after 3 months were measured. Result: The BMD content in the control group

model group

CEE3 group

low-dose

medium-dose

high-dose of Wenshen Tongluo Zhitong granule group was(0.231 8±0.003 6)

(0.198 8±0.003 9)

(0.219 9±0.002 4)

(0.201 1±0.000 9)

(0.202 3±0.002 7)

(0.220 2±0.002 1)g·cm-2

the CT content is (74.61±10.54)

(48.33±13.72)

(71.72±14.83)

(56.76±12.58)

(68.39±13.74)

(75.29±15.63) ng·L-1

the BGP content is (5.95±1.20)

(5.46±1.34)

(6.27±1.21)

(6.08±1.24)

(6.12±1.47)

(6.15±1.19) μg·L-1

the E2 content is (88.61±17.54)

(28.45±11.39)

(71.71±8.69)

(68.11±12.63)

(70.93±10.42)

(75.21±9.37) ng·L-1. The BGP content in ovariectomized rats had no significant change in the model group

treatment groups and CEE3 group compared to the control group. The BMD and CT content in treatment groups significantly increased compared with the model group

and the high dose group improved most significantly (P<0.01). The E2 content in treatment groups significantly increased compared with the model group(P<0.01). Conclusion: Wenshen Tongluo Zhitong granule can significantly increases bone mineral density and improve bone metabolism in ovariectomized rats

which plays the role of anti-osteoporosis.