Objective:To optimize extraction conditions of total saponins from Panax japonicus by foam separation technique. Method: Taking enrichment ratio

purity ratio and recovery as indexes

effect of pH

bubble size

temperature of mixture

electrolyte concentration and other main influencing factor on foam separation was investigated

single factor test was used to optimize extraction technology of total saponins from P. japonicus by foam separation. Result: Optimum extraction conditions were as followings:bubble diameter 0.4-0.5 mm

temperature 65℃

pH 5.6

the concentration of NaCl 0.015 mol·L-1

under these conditions

enrichment ratio of total saponins was 2.1

purity ratio was above 2.6 and recovery was 98.33%. Conclusion: Foam separation technique could be applied to separate total saponins from P. japonicus with stability

reliability and high yield

it provided a reference for development and utilization of natural total saponins in P. japonicus.