Objective: To optimize purification technology conditions of alkaloids from Strychnos nux-vomica in Yisui Tongjing prescription by macroporous adsorption resin. Method: With the content of brucine and strychnine as indexes

which were determined by HPLC

macroporous adsorption resin type was screened by static adsorption and desorption test

single factor test was used to investigate adsorption and elution conditions

such as adsorption speed

adsorption time

elution concentration

elution speed

the amount of elution and so on

to determine purification technology of alkaloids from S. nux-vomica in Yisui Tongjing prescription. Result: Optimum purification technology was:type of macroporous resin was X-5

the concentration of sample solution 0.1 g· mL-1

loading speed 1.0 mL· min-1

sample volume 4 BV

adsorption time 2 hours

washed with 1 BV water for eluting impurity

eluted with 6 BV 50% ethanol

elution rate 2.0 mL· min-1. Conclusion: Optimized technology was simple and economical

it was feasible for taking macroporous adsorption resin to purify alkaloids from S. nux-vomica.