Objective: To optimize preparation technology of β-cyclodextrin inclusion compound of volatile oil from Baizhishan cold granule. Method: With inclusion rate of volatile oil

yield and oil ratio of inclusion complex as indexes

optimum technology was selected from saturated aqueous solution method

ultrasonic method and trituration method;With inclusion rate of volatile oil and yield of inclusion complex as comprehensive evaluation indexes

orthogonal design was used to optimize inclusion technology of volatile oil with ratio of volatile oil-β-CD

inclusion temperature

inclusion time and proportion of water-β-CD as factors;Phase of this prepared inclusion complex was testified by analyses of TLC

UV and microscopic imaging. Result: The best method of inclusion was saturated aqueous solution method

and optimum inclusion technology conditions were as follows:ratio of volatile oil-β-CD 1∶8

inclusion temperature of 55 ℃

mixing time of 3 h

β-CD-water ratio of 1∶10.Analysis results of TLC

UV and microscopic imaging showed that stable inclusion compound has been formed from volatile oil and β-CD. Conclusion: This optimized technology was simple

stable and feasible

it could be suitable for large-scale production.