HE Kui-bang, WANG Ying-zi, ZHANG Chun-ni, et al. Optimization of Water Extraction Technology for by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 33-35.
HE Kui-bang, WANG Ying-zi, ZHANG Chun-ni, et al. Optimization of Water Extraction Technology for by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 33-35.DOI:
Objective: To optimize water extraction technology conditions of Pheretima aspergillum. Method: With the content of hypoxanthine as index
based on single factor test
effects of extraction time
extraction times and the amount of water were investigated by L9(34) orthogonal test.HPLC was used to determine the content of hypoxanthine. Result: Influencing order of various factors on water extraction technology was extraction times>extraction time>the amount of water;Optimal water extraction technology was as following:reflux extracted 1 time with 10 times the amount of water
extraction time of one hour. Conclusion: This optimized technology was stable and feasible.