Objective:To optimize mixed proportion for prescription of oily acne herbs through response surface method. Method: Bacteriostasis experiment

hyaluronic acid and inhibiting thrombin experiment in vitro and inhibiting human sebum secretion experiment were carried out. Based on the results of these experiments

the indicators were analyzed by response surface method to find the best ratio finally. Result: Simulation and prediction of the best ratios of the herb extracts showed dandelion extract (1.19)

Magnolia bark extract (2.19) and ligusticumchuanxiong extract (2.24). The prediction estimated a relative-inhibiting compound fat rate of 38.9%. Because all three kinds of Chinese traditional medicine extract is 0.2 g·mL-1

extracts can be expressed as a proportion of their own quality of Chinese herbal medicines. The real experimental value of the relative-inhibiting compound fat rate was 40.25%. Conclusion: The predicted value of the relative-inhibiting compound fat rate though response surface method is very similar to the experimental value. Response surface method can be employed in the proportion study of compound prescriptions.