益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞表达影响

吴至久; 王飞

您当前的位置：

首页 >

文章列表页 >

益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞表达影响

更新时间：2024-01-04

- 益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞表达影响
- Effects of Yiqi Yangxue Wenyang Formula on Interstitial Cells of Cajal in the Rat colon of Constipation caused by Laxatives
- 中国实验方剂学杂志 2013年19卷第2期页码：219-222
- 作者机构：
- 作者简介：
- 基金信息：
  
  2009年四川省教育厅课题(09ZA021)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
吴至久, 王飞. 益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞表达影响[J]. 中国实验方剂学杂志, 2013,19(2):219-222.

WU Zhi-jiu, WANG Fei. Effects of Yiqi Yangxue Wenyang Formula on Interstitial Cells of Cajal in the Rat colon of Constipation caused by Laxatives[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 219-222.
吴至久, 王飞. 益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞表达影响[J]. 中国实验方剂学杂志, 2013,19(2):219-222. DOI：

WU Zhi-jiu, WANG Fei. Effects of Yiqi Yangxue Wenyang Formula on Interstitial Cells of Cajal in the Rat colon of Constipation caused by Laxatives[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 219-222. DOI：

摘要

目的: 通过研究益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞含量的影响

阐明益气养血温阳方治疗泻药性便秘的机制。方法: 大鼠按照200 mg·kg-1·d-1大黄免煎剂灌胃

再按每日200 mg·kg-1·d-1递增

至半数以上动物出现腹泻

如此反复用药3月

造成"泻药性便秘"动物模型。将60只SD大鼠随机分为空白对照组、阳性对照(福松1.65 g·kg-1)组、模型组、益气养血温阳方低、中、高剂量(8.35

16.7

33.4 g·kg-1)组。阳性对照组、模型组、益气养血温阳方低、中、高剂量组给予大黄建立便秘大鼠模型

阳性对照和益气养血温阳方低、中、高剂量组按剂量给予药物治疗2周

取大鼠结肠组织

采用RT-PCR方法检测结肠中Cajal间质细胞表达。结果: 益气养血温阳方高剂量组结肠组织Cajal细胞mRNA相对表达量为0.365 08±0.047

中剂量组为0.370 97±0.040

与模型组大鼠结肠组织中表达为0.298 55±0.046比较

有显著差异(P<0.05)。结论: 益气养血温阳方通过改变Cajal间质细胞表达

促进结肠运动

起到治疗便秘的作用。

Abstract

Objective: To study the effects and mechanism of Yiqi Yangxue Wenyang formula on interstitial cells of cajal in the rat colon of constipation caused by laxatives. Method: The rat constipation model was caused by laxatives on. First day rats were ig given rhubarb 200 mg· kg-1· d-1

second day given 400 mg·kg-1· d-1 for 3 months

until half rats appeared diarrhea. The modelling rats were treated with Yiqi Yangxue Wenyang formula for two weeks

interstitial cells of cajal in colon was observed by RT-PCR. Result: The interstitial cells of cajal in rat colon in the Yiqi Yangxue Wenyang formula high dose group was 0.365 08±0.047

the medium group was 0.370 97±0.040

that was higher than that in the model group is 0.298 55±0.046(P<0.05). Conclusion: The Yiqi Yangxue Wenyang formula can treat constipation by adjust interstitial cells of cajal in colon.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于生物信息学和实验验证探讨首荟通便胶囊治疗便秘的作用机制

便秘与肺炎因果关系的双向孟德尔随机化分析

中药调控便秘相关信号通路的研究进展

中医药治疗糖尿病胃肠病变的疗效及机制研究进展

便通胶囊/片治疗便秘临床综合评价