Objective: In this study

we aim to investigate mechanism of Ziying Qingre prescription on acne. Method: The animals were randomly divided into 4 groups

namely blank control group

positive control group

high and low dosage of Ziying Qingre prescription. They were respectively fed with saline solution

antisterone(10 mg·kg-1)

Ziying Qingre prescription(18

9 g·kg-1) for 4 weeks. We measured the greatest transverse and greatest longitudinal diameter of bilateral sebaceous gland plaques with vernier caliper under strong light. The size change (greatest transverse diameter multiply greatest longitudinal diameter)and histopathology of sebaceous gland plaques were observed. Result: The size of sebaceous gland plaques of high dosage group were decreased (P<0.05) compared to the blank control groupHistopathology showed Ziying Qingre prescription made the numbers of sebaceous gland layers decreased

gland atrophy

arrangmentloose. Conclusion: Ziying Qingre prescription can suppress the proliferation of sebaceous glandwhich may be one of the mechanisms in treatment of acne.