Objective:To optimize extraction and purification technology of total triterpene acid from Hedyotis diffusa. Method: With extraction rate of total triterpene acid as index

the amount and concentration of ethanol

reflux time were chosen as factors

extraction technology of total triterpene acid from H. diffusa was optimized by orthogonal test;Resin model was screened by static adsorption and desorption test

purification technology of total triterpene acid was investigated by single factor test. Result: Optimum extraction technology was:reflux extracted 1.5 h with 10-fold 70% ethanol.Optimum purification technology of AB-8 macroporous resin was:sample flow rate 2 BV·h-1

the concentration of sample liquid 0.25 g·L-1

pH 5

the concentration of elution was 80% ethanol

eluting velocity 2 BV·h-1 with pH 7. Conclusion: Optimized technology was simple

stable with good reproduciblity

which was suitable for industrial production of total triterpene acid.