Objective: To observe anti-adenovirus 3 (AdV3) effect and cytotoxicity of andrographolide sulfonated (ADS) in vitro. Method: The maximum nontoxic concentration (0% toxic concentration

TC0) and 50% toxic concentration (TC50) of ADS were analyzed by cytopathic effect (CPE) and methyl thiazolyl tetrazolium (MTT) colorimetric test. The AdV3 at 100TCID50(50% tissue cell infection dose) attacked Hep-2 cellin vitro culture to cause AdV3 infection model. The anti-adenovirus activity of ADS at 6.10

12.21

24.41

48.23

97.66

195.31

390.63

871.25 mg·L-1 in vitro were measured with ribavirin as control

and the minimum effective concentration (MEC)

50% effective concentration (EC50) and therapeutic index (TI) were used as the indicators. Result: ADS and ribavirin TC0 were 781.25 mg·L-1and 6.25 mg·L-1; MECs were 97.66 mg·L-1and 0.390 6 mg·L-1

TIs were 8 and 16

respectively. Conclusion: ADS 97.66-781.25 mg·L-1can effectively inhibit the intracellular proliferation of AdV3 in vitro

and showed a significant dose-effect relationship.