HPLC测定复方挥发油-环糊精包合物中桂皮醛、丁香酚、苍术素的含量

张军; 叶炳皇; 陈威; 贺倩; 强皎

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HPLC测定复方挥发油-环糊精包合物中桂皮醛、丁香酚、苍术素的含量

更新时间：2024-09-23

- HPLC测定复方挥发油-环糊精包合物中桂皮醛、丁香酚、苍术素的含量
- HPLC Determination of Cinnamaldehyde, Eugenol, Atractylodin in Inclusion Complex of Compound Oil--Cyclodextrin
- 中国实验方剂学杂志 2013年19卷第3期页码：86-89
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省省部产学研基金项目(2010B090400135);东莞国际合作基金项目(2011B050300021)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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张军, 叶炳皇, 陈威, 等. HPLC测定复方挥发油-环糊精包合物中桂皮醛、丁香酚、苍术素的含量[J]. 中国实验方剂学杂志, 2013,19(3):86-89.

ZHANG Jun, YE Bing-huang, CHEN Wei, et al. HPLC Determination of Cinnamaldehyde, Eugenol, Atractylodin in Inclusion Complex of Compound Oil--Cyclodextrin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 86-89.
张军, 叶炳皇, 陈威, 等. HPLC测定复方挥发油-环糊精包合物中桂皮醛、丁香酚、苍术素的含量[J]. 中国实验方剂学杂志, 2013,19(3):86-89. DOI：

ZHANG Jun, YE Bing-huang, CHEN Wei, et al. HPLC Determination of Cinnamaldehyde, Eugenol, Atractylodin in Inclusion Complex of Compound Oil--Cyclodextrin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 86-89. DOI：

摘要

目的: 建立复方挥发油β-环糊精包合物中桂皮醛、丁香酚、苍术素含量的HPLC测定方法。方法: 采用HPLC

色谱柱:Phenomenex C18柱(4.6 mm×250 mm

5 μm);流动相:以乙腈-水梯度洗脱;柱温为室温;检测波长:桂皮醛、丁香酚为230 nm

苍术素为340 nm;流速:1.0 mL·min-1。结果: 桂皮醛在0.110 2~1.102 0 μg呈良好线性关系(r=0.999 8)

平均回收率为100.6%

RSD 1.24%;丁香酚在0.213 6~2.136 0 μg呈良好线性关系(r=0.999 9)

平均回收率为99.8%

RSD 1.55%;苍术素在0.020 0~0.200 0 μg呈良好线性关系(r=1.000 0)

平均回收率为100.2%

RSD 1.74%。阴性对照无干扰。结论: 该方法操作简便

结果准确可靠

为复方挥发油β-环糊精包合物的稳定性研究奠定了基础。

Abstract

Objective:To establish the HPLC method for determination of cinnamaldehyde

eugenol

atractylodin in inclusion complex of compound oil-β-cyclodextrin. Method: The high performance liquid chromatography method was developed by a Phenomenex C18 column (4.6 mm×250 mm

5 μm). It was eluted with stepwise gradient of acetonitrile and water. The column temperature was kept at room temperature. The detection wavelength was set at 230 nm for cinnamaldehyde

eugenol

and then 340 nm for atractylodin. The flow rate was 1.0 mL·min-1. Result: The linear ranges of cinnamaldehyde

eugenol and atractylodin were within 0.110 2-1.102 0 μg (r=0.999 8)

0.213 6-2.136 0μg (r=0.999 9)

and 0.020 0-0.200 0 μg (r=1.000 0) as the average recovery were 100.6%

RSD 1.24% (n=6)

99.8%

RSD 1.55% (n=6)

100.2%

RSD 1.74% (n=6). The negative sample had no interference. Conclusion: This method is simple

accurate and suitable for its assaying. It can be used for stability study in production of Inclusion complex of compound oil-β-cyclodextrin.

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