Objective:To investigate the influence of L-theanine on amino acids neurotransmitter and GluRs in rats by acute exhaustion swimming and mechanism of L-theanine improves exercise capacity. Method:SD male rats were randomly divided into high

middle and low dose (400

200

100 mg·kg-1

ig) groups and normal control group

ig

(n=10

in each group). After one week

each group was started to give L-theanine for 30 days. The normal control group was given normal saline. On the day 31

rat exhaustive swimming time was measured. Meanwhile

Glu

Gly

GABA and Asp of corpus striatum in rats were determined by HPLC. Immunoprecipitation and immunoblotting were applied to analyze the level of NR2A and tyrosine phosphorylation. Result:Compared with the normal control group

swimming time in high and middle dose groups increased significantly . Level of Glu

NR2A

tyrosine phosphorylation showed obviously higher in high dose group than the normal control group·L-1

(134.1±3.6)

(123.2±4.4)

(P<0.05)]. High dose group also showed lower GABA(1 881.3±336.1)μmol·L-1 level than normal control group (2 237.1±198.4) μmol·L-1(P<0.05). Conclusion:L-theanine has the effect of improving exercise capacity in rats possibly through enhancing Glu

NMDAR2A

tyrosine phosphorylation level and inhibiting the synthesis or secretion of GABA in brain.