Objective: To investigate the chemical components of flavonoids of Gardenia jasminoides. Method: Various column chromatography were used in the isolation and purification

and physiochemical constant determination and spectral analysis were adopted to determine the chemical structures of flavonoids. Result: Twelve flavonoids were isolated from G. jasminoides

quercetin (1)

quercetin-3-O-β-D-glucopyranoside (2)

rutin (3)

5-hydroxyl-7

3'

4'

5'-tetramethoxyflavone (4)

5

3'-dihydroxyl-7

4'

5'-trimethoxyflavone (5)

5

7-dihydroxyl-3'

4'

5'-trimethoxyflavone (6)

5

7

3'-trihydroxyl-8

4'

5'-trimethoxyflavone (7)

5

7

4'-trihydroxyl-8-methoxyflavone (8)

5

7

4'-trihydroxyl-6-methoxyflavone (9)

5-hydroxyl-6

7

3'

4'

5'-pentamethoxyflavone (10)

5

7

3'

5'-tetrahydroxyl-6

4'-dimethoxyflavone (11)

5

7

4'-trihydroxyl-3'

5'-dimethoxyflavone (12). Conclusion: Compounds 6

8 were first isolated from this plant.